Year: 2016 | Month: February | Volume 6 | Issue 1

Microbiological Analysis and Antibiotic Sensitivity of Water for Wild Animals in Nandankanan Zoo, Odisha


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Abstract:

The incidence and prevalence of bacterial pathogens affecting zoo animals is increasing due to consumption of contaminated water containing the various persistent bacterial pathogens with increased antimicrobial resistance. The consumption of this antimicrobial resistance water causes transmission of several water borne bacterial diseases. Hence to save the lives of wild animals and to protect the ecological balance of our environment, a critical routine systemic analysis of supplied water with good monitoring practice and development of a database for routine screening of the water for captive animals is very much essential. So, the experiment was conducted to assess the microbial load in the form of CFU/ml and the identification of antibiotic resistant bacteria by antibiotic resistance test (ABST) in the supplied water from the enclosure pools at Nandankanan Zoo, Odisha. It was found that several bacterial isolates like E. coli, Pseudomonas spp, Salmonella and Corynebacterium spp are exclusively present in this contaminated water based on the cultural, morphological and biochemical characterization. Under antibiotic sensitivity test (ABST), tetracycline and sulfamethoxazole-trimethoprim was found resistant for E.coli in all the collected samples of wild animal species including birds and reptiles. Cephalothin and Sulfisoxazole were moderately resistance to E. coli in case of birds whereas Gentamycin and Neomycin were moderately resistance to the sample collected from Lions enclosure.



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